The Analysis of P16 Gene Deletion and Mutation in Esophageal Squamous Cell Carcinoma
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摘要: 目的 :通过对p16基因变异在ESCC中的临床病理意义的研究,为ESCC的临床防治提供可靠指标。 方法 :采用PCR-SSCP及DNA序列分析,检测69例ESCC的p16基因第一、二外显子突变。 结果 :69例ESCC中p16基因变异者33例(M组)与未变异者36例(N组)在淋巴结转移率和远处转移率方面差异有显著性(P<0.05),两组在患者年龄、性别、肿瘤长度和浸润深度方面差异无显著性(P>0.05)。 结论 :p16基因的变异可能是ESCC的晚期变化,它可作为判断ESCC恶性程度、发展及预后的一个重要指标,并且也为筛选高危临床病例提供客观依据。Abstract: Objective: To intensively investigate the clinicopathologic significance of the alternadons of p16 gene in esophageal squamous cell carcinoma (ESCC), so as to provide a reliable indicator and create a new way for the research on clinical prevention and treatment of ESCC. Methods : Sixtynine samples from the patients with ESCC were enrolled. PCR-SSCP and DNAsequencing were utilized to detect the mutation and deletion of p16 gene exonl and exon2. Results : Thirty-three cases with p16 gene abnormality including sixteen point mutations and seventeen deletions, which were defined as Group M, were detected in sixty-nine cases of No p16 gene abnormality was found in the rest of thirty-six cases, which were classified as GroupN. There were significant differences in lymph node metastasis and distance metastasis between these groups (P<0.05). However, no significant differences in sex,age, the length and invasion’s depth of the tumor (P>0.05) were found. Conclusions : The mutations of p16 gene may be the late changes in ESCC. Detecting the mutation of p16 gene may serve as a marker of predicting degree of maligmancy, development and prognosis. It will render an authentic evidence for the selection of the clinical cases with high risk of metastasis.
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Keywords:
- Esophagus carcinoma p16 gene /
- Cene mutation /
- PCR-SSCP /
- DNA sequencing
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