The Effect of Intra-arterial Influsion Chemotherapy on Apoptosis and Proliferation in Squamous Carcinoma of the Cervix
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摘要: 目的:探讨介入化疗对宫颈鳞癌的细胞凋亡和增殖的影响.方法:选取宫颈鳞癌患者30例,介入化疗前后子宫组织,采用DNA缺口原位末端标记法(TUNEL)检测细胞凋亡,用凋亡指数(AI)表示;免疫组化染色SP法检测Ki67指数表达增殖变化,用LI表示;计算凋亡/增殖比例(AI/LI),并取30例正常宫颈上皮组织作为对照.结果:1)介入化疗后AI明显高于介入化疗前(P<0.05).2)介入化疗后LI明显低于介入化疗前(P<0.05).3)介入化疗前AI/LI(0.1293±0.079)低于对照组(0.2033±0.055),介入化疗后AI/LI(0.995 6±0.976)高于对照组,介入化疗后AI/LI明显高于介入化疗前,均有统计学意义(P<0.05).结论:介入化疗能诱导宫颈癌细胞的凋亡,抑制宫颈癌细胞的增殖;AI/LI能表示细胞凋亡和增殖之间平衡.Abstract: Objective :To investigate the effect of intra-arterial influsion chemotherapy on apoptosis and proliferation in squamous carcinoma of the cervix. Methods :Cervical tissues in normal cervix group, cervical cancer group before intra-arterial influsion chemotherapy and after intra-arterial influsion chemotherapy were examined and 30 cases were involved in each group. Apoptosis index (AI) was detected by DNA in situterminal leoxynucleotidyl transferase mediated nick end labeling (TUNEL).67 labeling index (LI) was detected by SP immunohistochemical technique. The rate of AI/LI was analyzed. Results : 1) Apoptosis index (AI) in cervical cancer group before intra-arterial influsionchemotherapy was significantly lower than that after intra-arterial influsion chemotherapy (P<0.05). 2)67 labeling index (LI) in cervical cancer group befc)二intra-arterial influsion chemotherapy was significantly higher than that after intra-arterial influsion chemotherapy (P<0.05). 3) The rate of AI/LI in cervical cancer group before intra-arterial influsion chemotherapy was lower than that in normal cervix group, the rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy was higher than that in normal cervix group. The rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy was higher than that before intra-arterial influsion chemotherapy (P<0.05). Conclusions :Interventional chemotherapy can induce apoptosis of human cervical cancer cells and inhibit the proliferation. The rate of AI/LI can express the balance of cell apoptosis and proliferation.
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