Abstract: Objective:To investigate the feasibility of detecting TLS/FUS-CHOP fusion gene transcript in paraffin-embedded tissue and its diagnostic value for myxoid liposarcoma. Methods: Six archival paraffin-embedded samples of myxoid liposarcoma were selected. Six cases of tumor with myxoid feature (including myxoid lipoma, lipoblastoma, myxoma, extraskeletal myxoid chondrosarcoma, myx-ofibrosarcoma)were used as negative control. 3-actin and 3z-microglobulin were used as internal control. Nested reverse transcription-polymerase chain reaction (RT-PCR)-based assay was performed to detect the TLS/FUS-CHOP fusion transcript. Results: 3-actin and 3Z-microglobulin were detected in 4/6 and 5/6 myxoid liposarcomas, respectively. The type II TLS/FUS-CHOP fusion transcript was found in 3 of 5 myxoid liposarcomas. 6 negative controls did not show detectable chimeric messages. Conclusion: Detection of TLS/FUS-CHOP fusion transcript in paraffin-embedded material by nested RT-PCR is feasible and is useful for the diagnosis and differential diagnosis of myxoid liposarcoma.