Abstract: Objective :To explore the effects of activation of TrkB on the chemotherapy resistance in neurobiastoma. Methods :The lack of human NB cell line SH-SY5Y expression of TrkB and BDNF were chosen,the expression of TrkB induced by ATRA was applied;activated the TrkB with BDNF and detected the survival and apoptosis rate of the different concentration cisplatins on the human NB cell line SH-SY5Y before and after the activation of TrkB with ATT and FCM. Results :There was no significant difference in survival rates of SH-SY5Y cells between BDNF+cisplatin and cisplatin alone (P>0.05) and no significant difference between ATRA+cisplatin and cisplatin alone (P>0.05),too. The survival rate of SY5Y cells in the groups of ATRA+BDNF+cisplatin (2.5~20ug/ml)was significantly higher than in the group with cisplatin alone (P<0.01);The apoptosis rate in the group of ATRA+BDNF+cisplatin was significantly lower than in the group of ATRA+cisplatin (P<0.01). Conclusions :The activation of TrkB can block the antitumor effect of cisplatin based on its inhibiting apoptosis of SH-SY5Y and may play an important role in madiating chemotherapy resistance in NB cell.