The Investigation on Relative Mechanism of Action between the Change of Intracellular Ca2+ and the Protease Caspase3 and Caspase8 in Apoptotic Process of Breast Cancer
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摘要: 目的: 探讨不同途径给药乳腺癌BCML-TA299移植瘤组织中Ca2+的变化与Caspase3、Caspase8蛋白相互作用的机制。 方法: 用а-干扰素不同给药途径处理BCML-TA299移植瘤组织。采用流式细胞仪、免疫组化技术分别测定乳腺癌BCML-TA299细胞的DNA含量、细胞周期变化及细胞内钙离子浓度水平变化与Caspase3、Caspase8蛋白表达相关性。 结果: 细胞形态学观察显示瘤内注射组出现典型的凋亡改变。瘤内注射组胞内Ca2+和Caspase3、Caspase8蛋白表达明显高于皮下注射组和预防注射组(P<0.01)。 结论: 1)Caspase3、Caspase8和胞内Ca2+共同参与了乳腺癌BCML-TA299细胞的凋亡调控过程,可能在乳腺癌发生、发展中起重要作用。2)不同途径给药对肿瘤细胞Caspase3、Caspase8表达和Ca2+浓度可产生不同的影响。Abstract: Objective : To investigate the change of Ca2+ and the mechanism of cooperation between the change of Ca2+ and the protease CaspaseD and Caspase8 in BCML - TA 299 breast cancer tissues treated by different methods of giving interferon-a (IFN-a). Methods : At the begining 60 mice were divided into four groups: the intratumoral injection group, subcutaneous injection group, preventive injection group and the control. BCML - TA299 breast cancer tissues was treated by different ways of administration using IFN-a. The DNA content, change of cell cycle and the relationship between the change of Ca2+ level and the expression of Caspasse3 and Caspase8 protein were tested. Results : After injection of INF-aby various ways of administration in different groups, the breast cancer cell in each group with BCML - TA299 was observed by method of cell morphology. There was an obvious change of apoptosis in intratumoral injection group. Ca2+ showed a very higher level in intratumoral injection group than in the subcutaneous injection and preventive injection group during the apoptotic process.The protease Caspase3 and Caspase8 played an important roles in physical regulation of Ca2+ in cell apoptotic process. Conclusions : a) The protease Caspase3 and Caspase8 and Ca2+ in cell cooperate in process of regulating the apoptosis in BCML - TA299 breast cancer tissues; b) Different ways of administration can produce various effect on the expression of Caspase3 and Caspase8 and the change of Ca22+ in tumor cells.
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Keywords:
- Breast cancer /
- Ca2+ /
- Caspase3 /
- Caspase8
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