Abstract: Objective : To study the inhibitory effect of celecoxib on proliferation of two human lung cancer cells (Anip973、AGZY83-a) and the effect on cell cycle, attachment, invasion and chemotaxied-motion Methods : Methyl thiazolyl tetrazolium (MTT) assay was used to observe the growth inhibition rate of human lung cancer cell line Anip973, AGZY83- a that were exposed to the various concentration of the celecoxib (5, 10, 20, 40, 80 and160μmol/L) at the different times. Cell cycles phase distribution of cells was measured by flow cytometry(FCM) assay. The ability of attachment to Lamininin and ECM gel was measured by MTT assay. The ability of invasion and of chemotaxied- motion through reconstituted basement membrane were investigated in transwell chambers. Results : Celecoxib could inhibit the growth of Anip973, AGZY83-a. Effects of inhibition depended on the action time and a certain concentration of celecoxib (P<0.01). Two cells after treated by celecoxib showed morphological changes characteristic of cells. Celecoxib (40μmol/L )could reduce abilities of attachment, invasion and chemotaxied -motion in lung cancer cells significantly (P<0.01). conclusion : Celecoxib with certain concentration can inhibit the proliferation of human cancer cells in concentration -depended and timedepended patterns and can reduce ability of attachment, invasion and chemotaxied-motion in lung cancer cells.