Abstract: Objective :To study the role of the MRP-1/CD9 and HSP60 in the development of bladder carcinoma. Methods :Expression of MRP-1/CD9 and HSP60 was detected by SP immunohistochemical technique in 75 cases of transitional cell carcinomas of the bladder (33 grade 1 tumors; 27 grade 2 tumors; 15 grade 3 tumors; 35 stage Ta tumors; 29 stage T, tumors; 11 stage TZT3 tumors), 15 cases of normal bladder tissues. The selected patients had a mean follow-up of 32 months (range,l2-49 months). Results : in normal bladder specimens, CD9 and HSP60 were expressed strongly in the membrane and cytoplasm respectively from bladder mucous membrane.the reduced/negative CD9 and HSP60 expression in tumor was detected respectively 41.2% and 42.7%. loss of CD9 expression was found to be associated with tumor grade and stage (P<0.05), A reduced/negative CD9 and HSP60 expression wasassociated with the risk of developing an infiltrating recurrence (P<0.05). Conclusions : Loss of CD9 and HSP60 expression may have prognostic relevance in patients with bladder carcinoma. CD9 and HSP60 may be used as a very useful maker for patients with superficial bladder carcinoma and may be used for predicting disease's progression.