The Research on Expression of the COX-2 in Various Subtypes of Gastric Mucous Membrane Intestinal Metaplasia and Gastric Cancer Using Tissue Microarray
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摘要: 目的: 研究环氧合酶-2(cyclooxygenase-2,COX-2)在不同亚型胃粘膜肠化生(intestinalmetaplasia,IM)及胃癌中的表达,探讨其预测IM恶变趋势的可能性,同时探讨COX-2表达与胃癌发生间的关系。 方法: 选择40例慢性萎缩性胃炎(chronicatrophicgastritis,CAG)伴IM、40例胃癌及相应癌旁组织,构建组织芯片。分别用高铁二铵/爱先蓝(HID/AB)及HE染色对IM及胃癌进行分型,然后用免疫组化检测不同亚型IM及胃癌中COX-2蛋白的表达。 结果: COX-2表达阳性率在CAG伴IM灶、癌旁IM灶、肠型胃癌中分别为60.87%、75.00%和86.36%,三者间无显著性差异,但表达强度在CAG伴IM灶→癌旁IM灶→肠型胃癌顺序中呈逐渐升高趋势(P<0.005)。肠型胃癌中COX-2表达阳性率及强度均显著高于弥漫型胃癌(P<0.005)。Ⅲ型IM中COX-2表达阳性率显著高于Ⅰ、Ⅱ型IM(P<0.05),从Ⅰ型、Ⅱ型到Ⅲ型,COX-2表达强度也呈逐渐升高趋势(P<0.005)。 结论: 随着IM愈倾向于恶性,COX-2表达水平也逐渐增高,其有可能成为预测IM恶变趋势的有用指标。COX-2表达主要与肠型胃癌的发生有关,但在弥漫型胃癌的发生中可能也有一定作用。Abstract: Objective :To study the expression of cyclooxygenase-2 (COX-2) protein in different subtypes of intestinal metaplasia (IM) of the gastric mucous membrane and gastric carcinoma, explore the possibility of forecasting the risk of malignant potential of IM with COX-2, and to investigate therelationship between COX-2 expression and gastric carcinogenesis. Methods :A total of 40 cases of chronic atrophic gastritis (CAG) with IM, 40 cases of gastric carcinoma, and corresponding paracancer-ous tissues were selected, respectively, in order to construct a tissue microarray. High iron diamine /alcian blue (HID/AB) staining and Hematoxylin and Eosin (HE) staining was used to classify IM and gas-tric carcinoma, and the expression of COX-2 protein was assessed using immunohistochemistry. Results :The positive rate of COX-2 expression was 60.87%, 75.00%, and 86.36% in IM foci in CAG, IMfoci in paracancerous tissues, and the intestinal-type gastric carcinoma, respectively, and there was no significant difference among them. However, the expressional intensity of COX-2 protein showed agradually increased tendency in the sequence of IM foci in CAC;IM foci in paracancerous tissues-intestinal- type gastric carcinoma (P<0.005). The positive rate and intensity of the expression of COX-2protein in the intestinal- type gastric carcinoma wire all significant higher compared to the diffuse-type gastric cancer (P<0.005). The expressional positive prevalence of COX-2 protein in type III IM was significant higher than that in type I and type II (P<0.05), and the expressional intensity also showed a gradually increased tendency from type I to type III (P<0.005). Conclusions : The expressional level of COX-2 increases gradually with the increase of the risk of malignancy of I and its expressional level may be a useful index to forecast the risk of malignant potential of IM. COX-2 expression is associated with the intestinal- type gastric carcinoma, but it might also have some role in the carcinogenesis of the diffuse- type.
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Keywords:
- Cyclooxygenase- 2 /
- Intestinal metaplasia /
- Gastric carcinoma /
- Tissue microarray
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