Abstract: Objective: To explore positive expression rate of c-kit (CD117, tyrosine kinase) in 32 cases of uterine sarcomas (20 leiomyosarcomas, 10 endometrial stromal sarcomas and 2 adenosarcomas) to further confirm whether uterine sarcomas express CD117, like gastrointestinal stromal tumors, and to seek new approaches for the treatment. Methods: CD117 was detected in 32 cases of uterine sarcomas with immunohistochemical staining. Five cases of gastrointestinal stromal tumors, diagnosed with pathological evaluation and CD117 positive expression were choosen as positive control. Results: Positive rates of CD117 were as follows: 11/20 (55%) leiomyosarcomas, 3/10 (30%) endometrial stromal sarcomas and 0/2 adenosarcomas. The total positive rate of uterine sarcomas was 14/32 (43.7%). Conclusion: Theoretical foundation is laid for choice of molecularly-targeted therapy for CD117 positive in unresectable or metastatic uterine sarcomas.