Abstract: Objective: To confirm the expression and distribution of aquaporin 1 (AQP1) andaquaporin 4 (AQP4) in laryngeal carcinoma and to probe into its significance in the development of la-ryngeal carcinoma.Methods: The tissues in a total of 20 cases of laryngeal carcinoma and paraneo-plastic issues of 15 cases were obtained immediately after surgery. The expression and distribution ofAQP 1 and AQP 4 in laryngeal carcinoma were examined by RT-PCR, Western Blot analysis and im-munohistochemical technique.Results: AQP1 expressed on capillary endothelia and laryngeal mucousglands of normal tissues, and expressed mainly on capillary endothelial cells, part of the tumor cellsand cancer nests of laryngeal carcinoma tissues. AQP4 expressed mainly on squamous epithelial, pseu-dostratified ciliated columnar epithelia and laryngeal mucous glands of normal laryngeal tissues and ontumor cell membrane and cancer nests of laryngeal carcinoma tissues.Conclusion: The distribution ofAQP 1 and AQP 4 on laryngeal carcinoma tissues is dramatically different and may increase water os-motic transport cooperatively in tumor epithelium and vessels, thus promoting the development and themetastasis of tumors.