Abstract: Objective: To study the morphology and distribution of vasculogenic mimicry in sec-tions of human tumor and animal model with melanoma by immunohistochemical staining and trans-electron microscopy.Methods: Tumors where vasculogenic mimicry appeared were stained with PASand CD31 to study the morphology and distribution. The active carbon dilution was injected into thecirculation system of B16-bearing mice. Tumor tissue where vasculogenic mimicry appear was observedwith electronic microscopy.Results: Vasculogenic mimicry where PAS was positive but CD31 was neg-ative in tumor tissues was not uniform and appeared in patches. There were three patterns of circula-tion, vasculogenic mimicry, vessels and endothelium cell-depended vessels. The result of animal exper-iment and electron microscopy validated the presence of vasculogenic mimicry.Conclusion: The resultobtained using active carbon as trace has proved that VM connect with the blood circulation. as thenew mode of tumor blood supply, vasculogenic mimicry exists in malignant tumors. The tumor cell canobtain oxygen and nutriment through vasculogenic mimicry.