Abstract: Objective: To analyze Ras-Raf-ERK1/2-mediated functions of bFGF on the mRNA expression of cyclin E and proliferation in Bel-7402 liver carcinoma cell and to investigate the signal transduction mechanism of proliferation in liver cancer. Methods: After treatment with bFGF, the cellcycle distribution, ERK1/2 activity and the mRNA expression of cycln E of Bel-7402 cell were studied using FACScan flow cytometry, Western blotting and RT-PCR respectively. Results: FCM analysis showed that bFGF induced S-phase entry (27.49± 0.72%→42.45± 1.06%) and bFGF, in a time anddose-dependence way, induced ERK1/2 activity and cyclin E mRNA expression. Treated cells with 25ng/ml bFGF, ERK1/2 activity and cyclin E mRNA expression reached the peak at 10min(3.84-fold in-duction) and 8h (5.15-fold induction), respectively. All the effects of bFGF were inhibited by PD98059, the inhibitor of MEK1. Conclusion: In Bel-7402 liver cancer cell, Ras-Raf-ERK1/2 path-way mediates the induction of cyclin E mRNA and the promotion of cell cycle resulted from bFGF. The bFGF signal transduction might play important roles in proliferation of liver cancer.