Abstract: Objective: To investigate the expression of the Signaling adapter protein PINCH in la-ryngeal squamous carcinoma and the impact of hypoxia on the expression of PINCH in laryngeal cancercell line Hep2. Methods: The specimens from 62 cases with metastasis of lymph node lesions and 22without metastasis of laryngeal squamous cancer were stained using ABC immunohistochemistry to de-termine the expression of PINCH. The normal laryngeal mucosa obtained from safe margin of thesecases were used as control. Human laryngeal cancer cell line Hep2 was divided into 3 groups: the hy-poxia groups for 12h and 24h, and the normoxia group for control. Flow cytometry (FCM) was used tomeasure the expression of PINCH in Hep2 cells. Results: a) The PINCH-positive expression rate of the carcinomatous tissue and normal laryngeal mucous tissue was 59.5% and 10% respectively, while in themetastasis group and non-metastasis group, the positive rates were 67.7% and 36.4%. There was a sta-tistically significant difference between the two groups (P<0.05); b) The expression of PINCH in Hep2cells affected by hypoxic condition was much higher than that in normal condition, which were detectedby FCM.(P<0.05). Conclusions: PINCH is up- regulated in laryngeal squamous cancer cells, and maybe correlate with the tumor's invasiveness and metastasis. Hypoxia may play an important role in the mechanism of up-regulating expression of the PINCH.