Abstract: Objective: To establish a hypoxic model of Hep2 laryngeal cancer cell line, and to in-vestigate the impact of hypoxia on cell cycle and Cisplatin (DDP)-induced apoptosis in Hep2 cells.Methods: Human laryngeal cancer cell line Hep2 was diviced into 4 groups: group A (contral), group B(hypoxia), group C (normoxia plus DDP) and group D (hypoxia plus DDP). Flow cytometry (FCM) wasused to measure the distribution of cell cycles and the protein levels of HIF-1αand to detect cellapoptosis. The rate of inhibition (IR) by DDP on Hep2 was determined using MTT. HIF- 1αmRNAlevel was determined by RT-PCR. Results: Hypoxia evidently increased the protein level of HIF-1α inHep2 cell, but had no effect on mRNA level of HIF-1α . Hypoxia could cause G ₀ /G ₁ cell cycle arrest inhuman Hep2 cell. The apoptosis and inhibition rate in group C were significantly higher than that ingroup D after treatment of DDP (P<0.05). Conclutions: Hypoxia could induce G ₀ /G ₁ cell cycle arrestand weaken the effect of DDP on inducing apoptosis of Hep2 cells.