The Expression and Significance of EphA2 Gene in Esophageal Carcinoma Tissues
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摘要: 目的 :探讨EphA2基因(包括mRNA和蛋白水平)的表达与食管癌发生及浸润转移的关系。 方法 :采用RT-PCR及免疫组化方法,检测食管癌组织及其正常粘膜中EphA2mRNA及蛋白的表达。 结果 :EphA2mRNA的表达在食管癌组织和其正常粘膜中未见显著性差异(P>0.05),且与肿瘤的分化程度、浸润深度及淋巴结转移无关(P>0.05)。EphA2蛋白在食管癌组织中的表达明显高于其正常粘膜(P<0.05),在深层浸润者的表达明显高于浅层浸润者(P<0.05),在有淋巴结转移者中的表达明显高于无淋巴结转移者(P<0.05),但与肿瘤的分化程度无关(P>0.05)。 结论 :EphA2蛋白的高表达可能与食管癌的癌变及侵袭转移的发生有关,但与EphA2mRNA的表达无关,推测这可能是由于翻译水平上调或蛋白质稳定性增高所致。Abstract: Objective :To investigate the relationship between EphA2 gene and the occurrence, Aswell as invasion and metastasis of esophageal carcinoma. Methods :The expression of EphA2 mRNAwas detected using RT-PCR and the EphA2 protein was detected by immunohistochemical method (SP)in esophageal cancer tissues and their normal mucosa tissues. Results :The expression of EphA2 mR-NA showed no difference between esophageal cancer tissues and normal mucosa, and it did not ap-peared to be correlated with differentiation of cancer tissues, the depth of infiltration and lymph nodemetastasis (P>0.05). The expression of EphA2 protein was significantly higher in cancer tissues than innormal mucosa tissues (P<0.05). The expressions of EphA2 protein in the deeper invasion group andthe group with lymph- node metastasis were significantly higher than those in the superficial invasiongroup and the group without lymph node metastasis (P<0.05). It did not appeared to be correlated withdifferentiation of cancer tissues (P>0.05). Conclusion :The occurrence of esophageal carcinoma and theformation of invasion and metastasis may be related to the overexpression of EphA2 protein but not tothe level of mRNA,which may results from the up-regulation in translational level or the increased pro-tein stability.
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Keywords:
- Esophageal neoplasm /
- EphA2 gene /
- RTKs /
- Metastasis
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[1] 张晓光,药立波,苏成芝.酪氨酸激酶受体Eph亚族的研究进展[J].生物化学与生物物理进展,1999,26(1):15-19 [2] Coffman KT,Hu M,Carles-Kinch K,et al. Differential EphA2epitope display on normal versus malignant cells [J].Cancer Res,2003,63(22):7907-7912 [3] Nakamoto M.Eph receptors and ephrins [J].Int J Biochem &CellBiol,2000,32(1):7-12 [4] Zelinski DP,Zantek ND,Stewart JC,et al.EphA2 overexpressioncause tumorigenesis of mammary epithelial cells [J].Cancer Res,2001,61(5):2301-2306 [5] Straume O,Akslen LA.Importance of vascular phenotype by ba-sic fibroblast growth factor, and influence of the angiogenic factorsbasic fibroblast growth factor/fibroblast growth factor receptor-1and Ephrin-A1/EphA2 on melanoma Progression[J].Am J Pathol,2002,160(3):1009-1019 [6] Kinch MS,Moore MB,Harpole DH.Predictive value of theEphA2 receptor tyrosine kinase in lung cancer recurrence andsurvival[J].Clin Cancer Res,2003,9(2):613-618 [7] Miyazaki T,Kato H,Fukuchi M,et al.EphA2 overexpressioncorrelates with poor prognosis in esophageal squamous cell carci-noma[J].Int J Cancer,2003,103(5):657-663 [8] 罗超,田刚,许民辉.Eph受体和Ephrin 配体的研究进展[J].中国癌症杂志,2003,13(1):79-82 [9] 张晓光,药立波,苏成芝.Eph受体家族及其配体的信号转导途径及功能[J].生物化学与生物物理进展,2001,28(4):498-501 [10] Nantek ND,Azimi M,Fedor-Chaiken M,et al.E-Cadherin reg-ulates the function of the EphA2 receptor tyrosine kinase [J].CellGrowth Differ,1999,10(9):629-638 [11] Walker-Daniels J,Hess AR,Hendrix MJC,et al.Differentialregulation of EphA2 in normal and malignant cells [J].Am JPathol,2003,162(4):1037-1042
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