Abstract: Objective: To investigate the function of the PP1195 protein in hepatocarcinoma cell lines. Methods: In vitro translation was applied to express PP1195 and fibronectin (FN) gene products, and then an in vitro binding experiment was used to observe the interaction between the two proteins. A cell adhesion experiment was used to observe the effect of the expression of the PP1195 gene on the binding capacity for FN of Hep3B cells and Hep3B cells overexpressing PP1195. The cells were observed for their invasion potential after scoring the bottom of the fibronectin-coated flask. Results: We have shown that there was an interaction between the PP1195 protein and FN. Induction of the expression of PP1195 can significantly improve the binding capacity of Hep3B cells to FN. The infiltration and migration potentials of SMMC7721cells were also significantly increased. Conclusion: PP1195 is a novel gene whose product can interact with fibronectin and can increase invasion and migration in hepatocarcinoma cells.