Abstract: Objective: To prepare an expression vector containing hnRNPA2/B1 fusion protein in order to synthesize monoclonal antibody. Methods: The cDNA of hnRNPA2/B1 gene was cloned from the total RNA of human muscle cells by RT-PCR. After sequencing, the gene was cloned into the expression vector PGEX-4T-1 to construct a recombinant plasmid named PGEX-A2/B1. E. coli BL21 cells containing the expression plasmid were induced with IPTG. Results: The full-length cDNA of the hnRNPA2/B1 gene was successfully cloned and confirmed by DNA sequencing. A prokaryotic expression vector for the hnRNPA2/B1 gene was constructed, and the fusion protein was expressed in E.coli after IPTG induction. Conclusion: The hnRNPA2/B1 gene was successfully cloned and a recombinant plasmid PGEX-A2/B1 containing this gene was constructed. The fusion protein expressed in E.coli was identified as hnRNPA2/B1 by Western blotting.