Abstract: Objective: To analyze the correlativity between the high-risk human papillomavirus (HPV) infections and concomitant epithelial lesions of the cervix in the patients with vulvar condyloma acuminatum. Methods: The vulvar biospy, colposcopy and high-risk HPV-DNA test were conducted in the patients with vulvar condyloma acuminatum and sampling with cervical bioscopy for cervical histopathological diagnosis was performed concurrently, from May 2002 to April 2004. Results: In 418 cases with vulvar condyloma acuminatum diagnosed by pathology, 287 were positive high-risk HPV (+) infections of cervix, accounting for 68.66%of the totals (287/418), among which the incidence of cervical intraepithelial neoplasm (CIN) was 20.56% (59/287); the high-risk HPV(-) occurred in 131 of total cases, accounting for 31.34%, among which the incidence of CIN was 7.63% (10/131). The results of histopathological examination were as follows: no cervical infection was found in 167 cases (39.95%), cervical HPV infection occurred in 182 (43.54%), CIN-Ⅰ was found in 51 cases (12.20%), CIN-Ⅱ in 16 (3.83%), CIN-Ⅲ in 2 (0.48%). None of them was cervical cancer. The vulvar condyloma acuminatum with concomitant VINII~III was found in only one case. Conclusions: The mixed HPV infection and lesion of multiple positions may occur in the patients with vulvar condyloma acuminatum. The vulvar condyloma acuminatum can be regarded as a characteristic clinical symptom, suggesting the possibility of cervical high-risk HPV infection with concomitant CIN, and more high-risk of HPV infection will exist in the women at pregnancy. Special care of cervical lesion should be paid for those with a history of vulvar condyloma acuminatum.