Effects of Gossypol on Apoptosis in Myeloid Leukemia Cells
-
摘要: 目的:本实验拟通过棉酚对3种经典髓系白血病细胞系的研究,探索其治疗白血病的可能。方法:用细胞培养、四唑盐比色试验、形态学、DNA电泳等方法观察棉酚对白血病细胞的凋亡效应。结果:棉酚在浓度<6μmol/L时可选择地抑制白血病细胞的增殖、促进其凋亡,并表现出浓度依赖效应。结论:棉酚对髓系白血病细胞具有选择杀伤作用,表明其可能是一种高效低毒的抗白血病药物。Abstract: Objective: Nowadays, the main reatment on leukemia is traditional radiochemotherapy with serious side effects and discouraged long time effects. It is necessary to look for new drugs for leukemia therapy. To study the possibility to treat leukemia with gossypol, we investigated the effects of gossypol on apoptosis in 3 classic human myeloid leukemia cell lines. Methods: The cytotoxic activity of gossypol on leukemia cell lines were detected by means of MTT assay. Apoptosis was identified and analyzed by morphology, cell cycle, and DNA gel electrophoresis, etc. Result: Our data showed that gossypol could selectly suppress the proliferation and induce apoptosis in all the three myeloid leukemia cell lines in a dose dependent manner(>6μM). Conclusion: Gossypol could selectively induce apoptosis in the myeloid leukemia cell lines. It suggests that gossypol may be an ideal drug for leukemia therapy.
-
Keywords:
- Gossypol /
- myeloid leukemia /
- proliferation /
- apoptosis /
- drug effects
-
-
[1] 1 Fanci R, Casini C, Leoni F,et al. Related Articles, Incidence and management of proven and probable funGLl infections in patients with acute leukemia: a single center experience [J]. J Chemother, 2004,16(6): 557～ 560
2 Bader P, Niemeyer C, Willasch A. Children with myelodysplastic syndrome and increasing mixed chimaerism after allogeneic stem cell transplantation have a poor outcome which can be improved by pre- emptive immunotherapy [J]. Br J Haematol, 2005,128(5): 649～ 658
3 Bushunow P, Reidenberg MM, Wasenko J, et al. Gossypol treatment of recurrent adult malignant gliomas[J]. J Neurooncol, 1999, 43(1): 79～ 86
4 Chen XQ, Wan YF, Bai QX, et al. Sodium Salicylate- Triggered Apoptosis in HL- 60 Cells Depends on Caspase- 8 Activation[J]. International Journal of Hematology, 2002,75(4):407～ 411
5 张永清,黄高昇,陈协群,等.HL- 60 细胞定向分化时 Bcl- 6 的表达 [J].中华血液学杂志,2004,25(9):569～ 571
6 Oliver CL, Miranda MB, ShanGLry S, et al. Gossypol acts directly on the mitochondria to overcome Bcl- 2- and Bcl- X (L)- mediated apoptosis resistance[J]. Mol Cancer Ther, 2005,4(1):23～31
7 Coutinho EM, Athayde C, Atta G. Gossypol blood levels and inhibition of spermatogenesis in men taking gossypol as a contraceptive. A multicenter, international, dose- finding study [J]. Contraception, 2000,61(1):61～ 67
计量
- 文章访问数: 3
- HTML全文浏览量: 0
- PDF下载量: 1
下载:
