Abstract: Objective: To investigate the roles of tumor suppressor genes -PTEN, nm23H₁, VEGF₁₆₅, Tiam1, MMP-2, Timp2, HE4 and S100A4 in carcinogenesis and progression of the ovarian cancer. Methods: Different expression profiles of the genes in normal ovary (n=5) and ovarian cancer tissue (n=20) were observed by cDNA microarray. Results: The PTEN, Timp2 and nm23H₁ genes were down-regulated ovarian cancer (CY-3/CY-5<0.5) and the Tiam1, VEGF₁₆₅, MMP-2, HE4 and S100A4 genes were up-regulated (CY-3/CY-5>2.0). The HE4 gene was up-regulated remarkably (CY-3/CY5>5.0). Those genes were correlated with clinicopathologic staging of ovarian cancer (P<0.01). Conclusions: Those genes are closely related with carcinogenesis of the ovarian cancer. The cDNA microarray technology will be of significance to the molecular mechanism of carcinogenesis and metastasis of the ovarian cancer and to detection of the marker of specific molecule in the ovarian cancer.