Abstract: Objective: To explore the clinical value between the mammotome minimally invasive biopsy (MMIBS) and fine needle aspiration cytology (FNAC) for diagnosis of the breast cancer. Methods: A total of 64 cases with breast cancer were performed with the ultrasound-guided MMIBS and FNAC. The clinical value of the 2 diagnostic methods for breast cancer was evaluated. Results: The rate of ultrasonic visualization in diagnosis of the 64 patients was 100%, in which the positive rate of MMIBS was 96.8% and that of FNAC 76.5% (P>0.05). The pseudo-negative rate of MMIBS was 4.2% and FNAC 23.4% (P<0.05). The pseudo-positive rate for both the methods was zero. Conclusions: The FNAC is convenient, safe and minimally invasive, but has a lower positive rate and a higher pseudonegative rate. The MMIBS boasts the characteristics of minimal invasion, high rate of final diagnosis and lowrate of missed diagnosis and can be regarded as first choice in qualitation of the breast masses.