Abstract: To detect the expression of TIMP- 1 and TIMP- 2 in breast cancer and to explore the correlation between this expression and the clinical prognosis. Method: TIMP - 1 and TIMP- 2 mRNA and protein were detected in 183 patients with breast cancer using microarray and S- P immunohistochemical technique. Results: a) The expression levels of TIMP- 1 (61.2%) and TIMP- 2 (75.4%) in the breast cancer tissues were significantly higher than those in benign breast diseases (P<0.01). b) TIMP- 1 expression was correlated with tumor size (P=0.049) and histological grade (P=0.014). It was also significantly correlated with the clinical TNM staging and lymph node status, but it had no correlation with age, pathological type, or ER and PR status. TIMP- 2 was significantly correlated with clinical TNM staging and lymph node status, but had no correlation with age, tumor size, pathological type, or ER and PR status. c) The 10- year survival rate in the group with overexpression of TIMP- 1 and TIMP - 2 was higher compared to the group with lower expression of TIMP - 1 and TIMP - 2. Conclusion: Both TIMP- 1 and TIMP- 2 can serve as markers for prognosis of breast cancer patients.