Abstract: To study the curative effect of pcDNA3/AFP/angio/tk targeted fusion gene system on subcutaneous transplantation tumor of human primary hepatic carcinoma. Methods: The subcutaneous transplantation tumor model of nude mouse with human primary liver cancer was constructed and the nude tumor- bearing mice were divided randomly into five groups (6 each group), i.e., the control group, void- plasmid group, GCV group, pcDNA3/angio/tk group and pcDNA3/AFP/angio/tk group. Different plasmas were directly injected into tumor and GCV intraperitoneal injection was given simultaneously based on variety of the groups. Pathologic examination included detection of in situ apoptosis using TUNEL method and observation by transmission electron microscope for change of cell ultrastructure. Results: The results of pathologic examination indicated that intratumoral injection of pcDNA3 AFP/angio/tk gene could remarkably inhibit the growth of tumor. Determination by TUNEL and electron microscope could detect the cell apoptosis in pcDNA3/AFP/angio/tk group. Conclusion: pcDNA3/ AFP/angio/tk targeted fusion gene system would remarkably inhibit the growth of tumor, and the curative effect of fusion gene system of the pcDNA3/AFP/angio/tk is superior to pcDNA3/angio/tk fusion gene (P<0.05).