The Relationship between egr- 1 Expression, Cell Proliferation and Apoptosis in Human Gliomas
-
摘要: 目的:探讨胶质瘤细胞egr-1基因表达水平与肿瘤恶性程度、细胞增殖活性及凋亡程度的关系。方法:用原位杂交、原位细胞凋亡检测和免疫组化染色方法观察了73例不同级别的胶质瘤。结果:73例胶质瘤egr-1 mRNA和EGR-1蛋白阳性表达率均为100%,这两种阳性肿瘤细胞密度均随肿瘤恶性程度升高而相应增加,不同级别组间比较差异均有显著性(P<0.01)。73例胶质瘤增殖细胞核抗原(PCNA)阳性肿瘤细胞和凋亡肿瘤细胞检出率均为100%。随肿瘤恶性程度升高,PCNA阳性肿瘤细胞密度增加而凋亡肿瘤细胞密度减少,不同级别组间比较差异均有显著性(P<0.05～0.01)。经直线相关分析证实,egr-1 mRNA、EGR-1蛋白和PCNA阳性肿瘤细胞密度彼此间均呈显著性正相关(r=0.685～0.999,P<0.01),前三种阳性肿瘤细胞密度均与凋亡肿瘤细胞密度呈显著性负相关(r=-0.758～-0.775,P<0.01)。结论:egr-1基因表达水平对评价胶质瘤生物学行为有重要参考价值。胶质瘤细胞egr-1基因表达异常增加可能是促进肿瘤细胞增殖和抑制其凋亡的重要因素,并在胶质瘤发生及恶性进展过程中均起重要作用。
-
关键词:
- 胶质瘤 /
- 早期生长应答基因-1 /
- egr-1 /
- 细胞增殖 /
- 凋亡
Abstract: Objective: To investigate the relationship between the expression level of early growth response gene- 1 (egr- 1) in human glioma, and the tumor grade, cell proliferation and apoptosis of the tumors. Methods: Seventy- three human glioma specimens with different grades of malignancy were studied using in situ hybridization, in situ cell death detection (TUNEL method) and immunohisto-chemistry. Results: All of the 73 gliomas expressed egr- 1 mRNA and EGR- 1 protein (100%). Not only was the positive cell density of egr- 1 mRNA and EGR- 1 protein increased with the degree of tumor malignancy but also their expression differences were significant among groups of grade- Ⅰ to Ⅱ, Ⅲ and Ⅳ (P<0.01). Both the expression of proliferating cell nuclear antigen (PCNA) and tumor- cell apoptosis were detected in all of the 73 glioma cases (100%). The PCNA- positive tumor cells were increased but apoptotic tumor cells decreased with an enhancement of the tumor malignancy. There were significant differences among the glioma cases of various malignant grades (P<0.05~0.01). The positive cell density of egr- 1 mRNA expression, EGR- 1 protein and PCNA protein were positively correlated with one another (r=0.685~0.999, P<0.01). However, each one of them was negatively correlated with the number of apoptotic tumor cells (r=- 0.758~ - 0.775, P<0.01). Conclusion: These results suggest that the expression level of egr- 1 mRNA has potential value in the evaluation of biological behavior of gliomas. Overexpression of the egr- 1 gene in glioma cells could possibly promote cell proliferation, inhibit apoptosis and play an important role in the development and malignant progression of glioma.-
Keywords:
- Glioma /
- Early growth response gene- 1 /
- egr- 1 /
- Cell proliferation /
- Apoptosis
-
-
[1] 1 Thiel G,Cibelli G.Regulation of life and death by the zinc fingertranscription factor Egr- 1[J].JCell Physiol,2002,193(3):287~292
2 Baron V,Duss S,Rhim J,et al.Antisense to the early growth re-sponse- 1 gene (Egr- 1) inhibits prostate tumor development inTRAMP mice[J].Ann N Y Acad Sci,2003,1002:197~216
3 Liu C,Yao J,Mercola D,et al.The transcription factor EGR- 1directly transactivates the fibronectin gene and enhances attach-ment of human glioblastoma cell line U251[J].J Biol Chem,2000,275(27):20315~20323
4 Al- Sarraj A,Thiel G.Substance P induced biosynthesis of thezinc finger transcription factor Egr- 1 in human glioma cells re-quires activation of the epidermal growth factor receptor and ofextracellular signal- regulated protein kinase [J].Neurosci Lett,2002,332(2):111~114
5 Lucerna M,Pomyje J,Mechtcheriakova D,et al.Sustained ex-pression of early growth response protein- 1 blocks angiogenesisand tumor growth[J].Cancer Res,2006,66(13):6708~6713
6 Kaufmann K,Thiel G.Epidermal growth factor and platelet- de-rived growth factor induce expression of Egr - 1,a zinc fingertranscription factor,in human malignant glioma cells [J].J NeurolSci,2001,189(1~2):83~91
7 于士柱,黄 悦,李 莉,等.胶质瘤细胞端粒酶基因表达对其增殖及凋亡影响的研究[J].中国神经肿瘤杂志,2005,3(2):82~87
8 于士柱,浦佩玉,江德华,等.胶质瘤 c- myc 基因表达水平与其细胞增殖和凋亡关系的研究[J].现代神经疾病杂志,2003,3(1):22~25
9 于士柱,浦佩玉,江德华,等.胶质瘤细胞 bcl- 2 基因表达水平与其细胞增殖及凋亡关系的研究[J].中华病理学杂志,2000,29(1):12～15
10 Krones- Herzig A,Mittal S,Yule K,et al.Early growth response 1acts as a tumor suppressor in vivo and in vitro via regulation ofp53[J].Cancer Res,2005,65(12):5133~5143
11 Okamura H,Yoshida K,Morimoto H,et al.PTEN expressionelicited by EGR - 1 transcription factor in calyculin A- inducedapoptotic cells[J].J Cell Biochem,2005,94(1):117~125
12 Weisz L,Zalcenstein A,Stambolsky P,et al.Transactivation ofthe EGR1 gene contributes to mutant p53 gain of function [J].Cancer Res,2004,64(22):8318~8327
13 于士柱,浦佩玉,江德华,等.胶质瘤细胞 PDGFBB 自分泌环活性与肿瘤细胞 egr- 1 基因表达关系的研究[J].中华神经外科杂志,2000,16(4):235~238
计量
- 文章访问数: 11
- HTML全文浏览量: 0
- PDF下载量: 1
下载:
