Abstract: Objective: To investigate the clinical significance of clusterin protein expression in ovarian cancer and its association with tumor cell apoptosis. Methods: A tissue microarray (TMA) containing 86 cases of epithelial ovarian carcinoma was constructed. Immunohistochemistry (IHC) was used to examine clusterin protein expression and TUNEL staining was used to determine the apoptosis index.These data were used to determine the clinical significance in relation to clinicopathological data. Results: Using the ovarian cancer TMA, IHC and TUNEL staining were successfully conducted in 73 cases of ovarian cancer. Overexpression of clusterin protein was observed in 38 cases (52%). There was a significant difference in clusterin expression between tumors of different clinical stages (P<0.05) where the overexpression of clusterin was frequently seen in advanced stage tumors (Ⅱ-Ⅳ). In addition, a lower apoptosis index (AI) was observed in the epithelial ovarian carcinomas with overexpression of clusterin, while a higher AI was shown in most of the tumors with normal clusterin expression (P<0.05).Conclusions: Overexpression of clusterin protein in ovarian cancer is positively correlated with the tumor's clinical stage and is negatively correlated with tumor cell apoptosis.