TRAIL诱导神经母细胞瘤细胞凋亡中Caspase 8活性的变化

陆春伟, 佟海侠, 张继红, 张锦华

陆春伟, 佟海侠, 张继红, 张锦华. TRAIL诱导神经母细胞瘤细胞凋亡中Caspase 8活性的变化[J]. 中国肿瘤临床, 2007, 34(14): 781-784.
引用本文: 陆春伟, 佟海侠, 张继红, 张锦华. TRAIL诱导神经母细胞瘤细胞凋亡中Caspase 8活性的变化[J]. 中国肿瘤临床, 2007, 34(14): 781-784.
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Lu Chunwei, Tong Haixia, Zhang Jihong, . Change of Caspase 8 Activity in TRAIL-induced Apoptosis of Neuroblastoma Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(14): 781-784.
Citation: Lu Chunwei, Tong Haixia, Zhang Jihong, . Change of Caspase 8 Activity in TRAIL-induced Apoptosis of Neuroblastoma Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(14): 781-784.
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TRAIL诱导神经母细胞瘤细胞凋亡中Caspase 8活性的变化

  • 1.

    中国医科大学公共卫生学院卫生检验教研室, 沈阳市 110001;

  • 2.

    中国医科大学盛京医院血液研究室

详细信息
    通讯作者:

    陆春伟luchunwei2002@yahoo.com.cn

Change of Caspase 8 Activity in TRAIL-induced Apoptosis of Neuroblastoma Cells

  • 1.

    Section of Health Ecsomatics, College of Public Health, China Medical University, Shenyang

摘要

    摘要
  • 摘要: 目的 :探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导神经母细胞瘤细胞株CHP212细胞凋亡过程中Caspase 8活性的变化。 方法 :应用流式细胞仪检测TRAIL、Caspase 8抑制剂(zIETD-FMK)+TRAIL对CHP212细胞的诱导凋亡作用:应用比色法测定Caspase 8相对活性;应用透射电镜对凋亡细胞进行形态学观察。 结果 :CHP212细胞对TRAIL的诱导凋亡作用敏感,存在剂量依赖性。随TRAIL作用时间的延长,Caspase 8活性逐步升高,于作用16h达高峰;不同浓度TRAIL处理组Caspase 8相对活性随浓度增加而递增。zIETD-FMK能阻断Caspase 8的活化而抑制TRAIL对CHP212细胞的诱导凋亡作用。透射电镜可见到典型的细胞凋亡特征。 结论 :TRAIL通过Caspase 8信号传导通路诱导CHP212细胞凋亡并伴随Caspase 8活性增高。
    Abstract: Objective : To study the change of Caspase 8 activity in TRAIL-induced apoptosis of CHP212 neuroblastoma cells. Methods: The effects of TRAIL and Caspase 8 inhibitor zIETD-FMK+ TRAIL on the apoptosis of CHP212 cells were detected using flow cytometry. Relative Caspase 8 activ-ity was measured by colorimetric assay. Apoptotic cells were visually detected using a transmission electron microscope (TEM). Results : CHP212 cells were sensitive to TRAIL and this sensitivity was dose-dependent. The relative Caspase 8 activity in CHP212 cells increased gradually with prolonged exposure to TRAIL and with increased TRAIL concentration. The zIETD -FMK inhibitor could block the activation of Caspase 8 and diminish the TRAIL-induced apoptosis of CHP212 cells. Typical fea-tures of apoptosis were seen under TEM. Conclusion : TRAIL could induce the apoptosis of CHP212 cells through a Caspase 8 signal transduction pathway, with an increase in Caspase 8 activity.
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    • 参考文献(1)
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    7. Muhlethaler-Mottet A,Bourloud KB,Auderset K. Drugmediated sensitization to TRAIL-induced apoptosis in caspase-8-complemented neuroblastoma cells proceeds via activation of intrinsic and extrinsic pathways and caspase-dependent cleavage of XIAP,Bcl-xL and RIP[J].Oncogene,2004,(32):5415-5425.doi:10.1038/sj.onc.1207704.
    8. 佟海侠,张继红,马力. Caspase-8和DR5在TRAIL诱导神经母细胞瘤细胞凋亡中的作用[J].中国当代儿科杂志,2006,(04):327-330.doi:10.3969/j.issn.1008-8830.2006.04.018.
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    . 10. 佟海侠,张锦华,陆春伟. IFNγ对TRAIL诱导神经母细胞瘤细胞凋亡的调节作用[J].中国肿瘤临床,2005,(23):1321-1324.doi:10.3969/j.issn.1000-8179.2005.23.001.
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  • 收稿日期:  2007-01-12
  • 发布日期:  2007-07-30

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