Abstract: Objective: To investigate the expression of TRAIL, Survivin and HPV 16E6/E7, their interaction and functional role in the cervical pre-invasive lesions development, cervical carcinomas tu-morigenesis and tumor progression. Methods: A total of 89 samples were analyzed in this study includ-ing 15 cases normal cervical tissue, 47 cases of cervical intraepithelial neoplasia tissue and 27 casescervical carcinoma tumor tissues. The expression of HPV16E6/E7 、 Survivin 、 TRAIL among all sampleswas determined by RT-PCR and the apoptotic index (AI) of tumor cells was determined by TUNEL as-say. Result: The expression of HPV16E6/E7 and Survivin showed increasing tendency following the development of cervical lesions, on the contrary, the expression of TRAIL showed decreasing tendency.There was no statistical significant difference of HPV16E6/E7 expression related with different cervical carcinomas age groups, clinical stages, histology grades and histology types (P>0.05). The level of Sur-vivin expression showed statistical significant differences between different clinical stages and histology grades (P<0.05) and the level of TRAIL showed statistical correlation with histology grades (P<0.05). A-mong all cervical carcinomas tissues, the level of HPV16E6/E7 expression was positively correlated with the level of Survivin and negatively correlated with the expression of TRAIL, the level Survivin expression was negatively correlated with the expression of TRAIL. Conclusions: The level of HPV16E6/E7 expression is closely relative with the development of cervical lesions and is a predicatorof disease stage. The disordered expression of HPV16E6/E7, Survivin and TRAIL gene may play a syn-ergistic effect during the tumorigenesis and progression of cervical carcinoma. Combinational examiningthe expression level of above 3 genes can be a more reliable and accurate approach for earlier diagnosisof cervical carcinomas and judgment prognosis.