Abstract: Colorectal cancer, one of the most common cancer types in China, involves multiple complex pathophysiological processes. In-depth studies of glycomics, have revealed close relationships between alterations in protein glycosylation and the occurrence, progression, metastasis, and multidrug resistance of colorectal cancer. Changes in glycosylation can serve as important biomarkers and provide new assays for early diagnosis and targets for intervention. This article reviews the latest research into altered protein glycosylation in colorectal cancer, with the goal of improving patient prognosis and reducing drug resistance by identifying relevant sites of altered glycosylation.