Abstract: Objective: To explore the clinical diagnostic value of the combination of detecting aberrant p16promoter methylation, CT and carcinoembryonic antigen (CEA) measurements for lung cancer. Methods:Sixty-seven cases with sus-pected lung cancer underwent detection of aberrant promoter methylation of p 16gene in plasma, CT and measurement of CEA in serum.Results: Of the 67cases with suspected lung cancer,42cases were diagnosed with lung cancer and the oth -ers were diagnosed with benign lung disease. The diagnostic sensitivity, specificity and accurate value in assessing lung carcinomas were 52.4%,96.0% and 68.7% with aberrant promoter methylation of p16gene8 0.9%,80.0% and 80.6% with CT5 4.8%,76.0% and 62.7% with CEA measurements respectively and were 92.9% ,84.0% and 89.6% with combined de-tection of the three methods, respectively. Conclusion:The combination of the three methods p16gene aberrant promoter methylation, CT and CEA measurements may be helpful in improving the diagnosis of lung cancer and is the better choice for differentiating benign pulmonary lesions from malignant ones.