张秀梅, 刘超, 赵颂, 王翠瑶, 肖建英. 9-顺式维甲酸抑制甲状腺鳞癌细胞SW579增殖的作用机制[J]. 中国肿瘤临床, 2012, 39(11): 761-764. DOI: 10.3969/j.issn.1000-8179.2012.11.002
引用本文: 张秀梅, 刘超, 赵颂, 王翠瑶, 肖建英. 9-顺式维甲酸抑制甲状腺鳞癌细胞SW579增殖的作用机制[J]. 中国肿瘤临床, 2012, 39(11): 761-764. DOI: 10.3969/j.issn.1000-8179.2012.11.002
shu
Xiumei ZHANG, Chao LIU, Song ZHAO, Cuiyao WANG, Jianying XIAO. Mechanism of 9-cisRA in Inhibiting the Proliferation of Thyroid Squamous Cell Carcinoma Cell SW579[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(11): 761-764. DOI: 10.3969/j.issn.1000-8179.2012.11.002
Citation: Xiumei ZHANG, Chao LIU, Song ZHAO, Cuiyao WANG, Jianying XIAO. Mechanism of 9-cisRA in Inhibiting the Proliferation of Thyroid Squamous Cell Carcinoma Cell SW579[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(11): 761-764. DOI: 10.3969/j.issn.1000-8179.2012.11.002
shu

9-顺式维甲酸抑制甲状腺鳞癌细胞SW579增殖的作用机制

Mechanism of 9-cisRA in Inhibiting the Proliferation of Thyroid Squamous Cell Carcinoma Cell SW579

    摘要
  • 摘要:
      目的  观察9-顺式维甲酸(9-cis-retinoic acid, 9-cisRA)对甲状腺鳞癌细胞株SW579细胞RARβ、p21、CDK4、CyclinDl表达的影响, 进而探讨9-cisRA的抗癌作用机制。
      方法  分别以终浓度为1×10-7、1×10-6、5×10-6、1×10-5mol/L 9-cisRA作用SW579细胞, 各组细胞均在培养24 h后加药, 继续培养48 h。用RT-PCR方法检测SW579细胞RARβ、p21、CDK4、CyclinD1的mRNA表达; 用Western Blot检测SW579细胞RARβ、p21、CDK4、CvclinD1的蛋白表达。
      结果  9-cisRA作用后, RT-PCR检测结果表明, 经不同浓度9-cisRA处理的细胞RARB、p21的mRNA表达水平均显著上调; CDK4的mRNA表达水平无明显变化; CyclinD1的表达水平明显下调; Western Blot检测结果表明经不同浓度9-cisRA处理的细胞RARβ、p21蛋白表达水平均显著上调; CDK4蛋白表达水平无明显变化; CyclinD1蛋白表达水平明显下调。
      结论  9-cisRA在一定浓度范围内可能通过上调RARβ、p21的表达进而下调细胞周期蛋白CvclinD1的表达, 从而抑制细胞增殖。

     

    Abstract:
      Objectives  The current study aims to observe the effect of 9-cisRA in the expressions of RARP, p21, CDK4, and CyinDl genes in the thyroid squamous cell carcinoma cells(SW579) to explore its mechanism.
      Methods  The SW579 cells were treated with different doses of 9-cisRA(final concentration of 1 x 10-7 mol/L, 1×10-6 mol/L, 5x10-6 mol/L, and 1×10-5 mol/L).The drug was added in the cells of all groups 24 h after the culture.The cells were then cultured for another 48 h.The RARβ, p21, CDK4, and CyclinDl mRNA expression levels were determined via RT-PCR, whereas the expression of RARβ, p21, CDK4, and CyclinDl protein were determined using Western blot.
      Results  The mRNA expression of RARβand p21 increased dramatically after the cells were treated with 9-cisRA, whereas the expression of CDK4 did not increase.In addition, the mRNA expression of CyclinDl dramatically decreased, whereas the protein expression of RARβand p21 significantly increased.No distinct change in the protein expression of CDK4 was observed.The CyclinDl protein expression was dramatically reduced.
      Conclusion  The drug 9-cisRA can inhibit the proliferation of thyroid squamous cell carcinoma cells in a dose-dependent manner.The mechanism of the effect may be related to the increase of RARβand p21mRNA and protein expression, as well as the decrease of CyclinDl mRNA and protein expression.

     

    • HTML全文
    • 参考文献(10)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回