Effects of trichosanthin on apoptosis and cytoskeleton microtubule structure reconfiguration in lung cancer A549 cells
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摘要:
目的 探讨天花粉蛋白（Trichosanthin, TCS）对肺癌A549细胞株抑制增殖作用及诱导凋亡效应。 方法 用不同剂量的TCS处理肺癌A549细胞，采用MTT法检测TCS对细胞增殖的抑制作用，透射电镜观察细胞骨架微管的变化，流式细胞术检测细胞周期和细胞凋亡率。 结果 TCS使肺癌A549细胞呈凋亡型改变，细胞活性下降，且随着浓度的升高、时间的延长抑制率相应升高。 结论 药理剂量的TCS可抑制肺癌A549细胞的增殖和分化，并通过细胞骨架的变化影响A549细胞的功能。 Abstract:Objective This study aimed to explore the effects of trichosanthin on the proliferative inhibition and apoptosis induction in human lung carcinoma A549 cells. Methods A549 cells were treated with various concentrations of TCS. The inhibitory effects in proliferation were detected by the MTT method. The microfilament changes were observed by transmission electron microscopy. Apoptosis rate and cell cycle were determined by flow cytometry. Results A549 cells treated with TCS presented apoptotic changes and decreased cell activity. When the concentration increased and time was prolonged, the inhibition rate increased correspondingly. Conclusions Pharmacological doses of TCS inhibited the proliferation and differentiation in lung carcinoma A549 cells and affected the function in A549 cells by changes in the cytoskeleton. -
Key words:
- trichosanthin /
- lung cancer /
- apoptosis /
- cytoskeleton
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图 1 TCS对A549细胞株荧光观察
A. 0 μg/ml TCS；B. 20 μg/ml TCS；C. 40 μg/ml TCS；D. 80 μg/ml TCS
Figure 1. Effects of TCS on the apoptosis of lung cancer A549 cells observed under a fluorescent microscope
图 2 TCS对A549细胞株微管的影响
A. 0 μg/ml TCS，×3000；B. 0 μg/ml TCS，×5000；C. 80 μg/ml TCS，×5000
Figure 2. Effects of TCS on the microtubule structure of lung cancer A549 cells observed under electron microscopy
表 1 TCS对A549细胞增殖抑制率的影响
Table 1. Inhibition ratio of TCS at various times and concentrations on A549 cells
表 2 TCS对细胞周期的影响
Table 2. Effect on cell cycle of TCS at various concentrations on A549 cells
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[1] 郭继武, 马彬, 周慧银, 等.吉非替尼治疗非小细胞肺癌的meta分析[J].中国肺癌杂志, 2011, 14(4):351-357. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgfazz201104009Guo JW, Ma B, Zhou HY, et al. Gefitinib for Non-small Cell Lung Cancer: A metaAnalysis[J]. Chin J Lung Cancer, 2011, 14(4): 351-357. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgfazz201104009 [2] Wang BL, Su H, Chen Y, et al. A role for trichosanthin in the expansion of CD4CD25 regulatory T cells[J]. Scand J mmunol, 2010, 71(4):258-266. doi: 10.1111/j.1365-3083.2010.02372.x [3] Fang FF, Zhang CZ, Zhang L, et al. Trichosanthin inhabits breast cancer proliferation in both cell lines and nude mice by promotion of apoptosis[J]. Plos One, 2012, 7(9):1-11. https://ui.adsabs.harvard.edu/abs/2012PLoSO...741592F/abstract [4] Sha O, Niu J, Ng TB, et al. Anti-tumor action of trichosanthin, a typeribosome-inactivating protein, employed in traditional Chinese medicine:a mini review[J]. Cancer Chemo Phar, 2013, 71(6): 1387-1393. doi: 10.1007/s00280-013-2096-y [5] Li M, Li X, Li JC. Possible Mechanisms of Trichosanthin-Induced Apoptosis of Tumor Cells[J]. The Anatomical Record, 2010, 293(6): 986-992. doi: 10.1002/ar.21142 [6] Walsh MJ, Dodd JE, Hautbergue GM. Ribosome-inactivating proteins:potent poisons and molecular tools[J]. Virulence, 2013, 4(8): 774-784. doi: 10.4161/viru.26399 [7] 李莉, 闫言.受体相互作用蛋白激酶RIP1在细胞信号传导途径中作用的研究进展[J].基础医学与临床, 2011, 31(10):1165-1167. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jcyxylc201110021Li L, Yan Y. Progress of study on functions of RIP1signal transduction[J]. Basic & Clinical Medicine, 2011, 31(10):1165-1167. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jcyxylc201110021 [8] 胡艳, 陈锐深.中药治疗支气管肺癌的分子生物学研究概况[J].广西中医药, 2006, 29(2):63-66. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxzyy200602001Hu Y, Chen RS. Research on Molecular biology of Chinese medicine treatment of bronchial lung cancer[J]. Guangxi J of TCM, 2006, 29(2):64-66. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxzyy200602001 [9] 李文赟, 张磊, 李福龙, 等.抗肿瘤细胞周期蛋白依赖性激酶抑制剂的研究进展[J].国外医药抗生素分册, 2009, 30 (3):113-120. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gwyykssfc200903003Li WY, Zhang L, Li FL, et al. Research Advances in Anticancer Cyclin-dependent-kinase Inhibitors[J]. World Notes on Antibiotics, 2009, 30(3):113-120. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gwyykssfc200903003 [10] Schuyler SC, Wu YF, Kuan VJ. The Mad1-Mad2 balancing act-a damaged spindle checkpoint in chromosome instability and cancer [J]. Cell, 2012, 125(18):4197-4206. https://jcs.biologists.org/content/joces/125/18/4197.full.pdf [11] Dorn JF, Maddox PS. Kinetochore dynamics:how protein dynamics affect chromosome segregation[J]. Curr Opin Cell Biol, 2012, 24(1): 57-63. doi: 10.1016/j.ceb.2011.12.003 [12] Ricke RM, van-Deursen JM. Correction of microtubule-kinetochore attachment errors:mechanisms and role in tumor suppression [J]. Semin Cell Dev Biol, 2011, 22(6):559-565. doi: 10.1016/j.semcdb.2011.03.007 [13] Margaron Y, Fradet N, Côté JF. ELMO recruits actin cross-linking family 7(ACF-7)at the cell membrane for microtubule capture and stabilization of cellular protrusions[J]. Biol Chem, 2013, 288(2): 1184-1199. doi: 10.1074/jbc.M112.431825 [14] Jain V, Jain B, Tiwari P, et al. Nanosolvated microtubule-modulating chemotherapeutics:a case-to-case study[J]. Anticancer Drugs, 2013, 24(4):327-336. doi: 10.1097/CAD.0b013e32835ec414 [15] Furst R, Vollmar AM. A new perspective on old drugs: non-mitotic actions of tubulin-binding drugs play a major role in cancer treatment[J]. Pharmazie, 2013, 68(7):478-483. -
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