Abstract: Objective:To explore the expression of C-terminal binding protein 2 (CtBP 2) in human esophageal carcinoma and its relationship with clinicopathological parameters and survival. Methods:The expression levels of CtBP2 in eight cases of fresh frozen specimens of esophageal squamous cell carcinoma (ESCC) and the adjacent esophageal tissues were detected by Western blot. Immuno -histochemistry was used to detect CtBP 2 expression in 90samples of ESCC tissues and adjacent non-tumor tissues. Based on patient in -formation and follow-up data, the correlation of CtBP 2 expression with patients' clinicopathological characteristics and overall survival was further evaluated using Fisher's exact test and Kaplan- Meier method, respectively. Results: Immunohistochemistry and Western blot analysis showed that CtBP 2 expression in tumor tissues was higher than that in adjacent non-tumor tissues. CtBP2 expression was significantly correlated with histologic grade ( P=0.002) and depth (P=0.032). Kaplan-Meier analysis demonstrated that high CtBP 2 ex -pression was correlated with a short survival time. Conclusion:CtBP2 expression was upregulated in ESCC tissues, indicating that it may play a role in the oncogenesis and development of ESCC.