Abstract: Osteosarcoma is a clinically common primary malignant bone tumor. Its treatment is still not ideal because of the high degree of malignancy and early metastasis. Understanding the molecular mechanism and the biological characteristics of malignant osteosarcoma, and exploring the effective treatment have become the focus of osteosarcoma research at home and abroad. A long noncoding RNA, lncRNA, is involved in the regulation of a variety of cellular functions and plays an important role in the development of osteosarcoma. Recently, lncRNA has been increasingly reported in osteosarcoma research. It has been found to affect the proliferation, invasion, and migration of osteosarcoma cells by interacting with proteins, mRNAs, and miRNAs. This article reviews the research on lncRNA based on recent reports regarding its role in the molecular mechanism of osteosarcoma.