Abstract: Objective: To investigate the roles of p14^ARF, p16^INK4a and p53 gene in oncogenesis of non-small cell lung cancer (NSCLC) and interrelations of them. Methods: The expression of p14^ARF,p1)iNK4aand p53 gene was detected in NSCLC by immunohistochemistry analysis. Results: The positive rate of p14^ARF, p16^INK4a and p53 gene expression in NSCLC tissues was 72.5%, 50% and 52.5% respectively ,which was significantly different from that (95%, 92.5%, 2.5% respectively) in normal tissues adjacent to cancer (P<0.01). The expression level of p14^ARF and p53 gene was not significantly related to histological classification, TNM stage, cancer cell differentiation and lymph node involvement in NSCLC. There was a significant relationship (P<0.05) between cancer cell differentiation and the expression level of p16^INK4a which wasn't significantly related to histological classification, TNM stage and lymph node involvement yet. A inverse significantly relationship was detected between p14^ARF loss and p53 expression in the study (r₂=-0.475, P<0.01), but there was not the significant correlation between p14^ARF and p16^INK4a expression. The overpositive proportion of all p14^ARF positive cases in stage I was 41.7 W and 10% in stage Conclusion: The aberrance of p14^ARF, p16^INK4a and p53 gene might be associated with the oncogenesis of non-small cell lung cancer. p14^ARF and p53 gene might play roles in a same regulable pathway. The expression level of , p14^ARF, p16^INK4a and p53 gene might be referred as markers of non-small cell lung cancer.