Abstract: Objective : To study the mechanism of Ginshenoside Rg3 in anti-lymphatic metastasis of hepatoma by the observation of the apoptosis effect which may be induced in mouse. Methods : The transplanted hepatoma models with lymphatic metastasis were set up, and the apoptosis of the primary and metastatic tumors was analysed in the following five groups: Ginsenoside Rg3 pretreatment, Ginsenoside Rg3 treatment, combination of Ginsenoside Rg3 & cisplatin treatment, cisplatin treatment and control (saline) groups. Results :The morphologic change under the light and electric miocroscope showed that more apoptosis bodies appeared in Ginsenoside Rg3 pretreatment and Ginsenoside Rg3 treatment groups compared with those of the other groups (5/5, 4/5); The extent of cell necrosis in cisplatin treatment group was more obvious; while the apoptosis and cell necrosis presenting in combination group in between. Apoptosis peak was observed by FCM and apoptosis nodule was detected in Ginsenoside Rg3-treated groups; The main change in the control group was cell injury, and no apoptosis peak detected. Conclusions : Apoptosis is one of the mechanisms of Ginsenoside Rg3 in volving its the anti-lymphatic, metastasis effect.