RKIP与NF-κB在肺癌侵袭转移中的作用
Role of Raf Kinase Inhibitor Protein and Nuclear Factor-κB in the Invasion and Metastasis of Lung Carcinoma
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摘要: 目的：检测肺癌组织中Raf激酶抑制蛋白（raf kinase inhibitor protein ，RKIP）和核因子κ B（nuclear factor-κ B，NF-κ B）的表达，分析其与临床病理特征及预后的关系。方法：应用免疫组织化学方法（SP法）检测83例肺癌及65例癌旁正常肺组织中RKIP与NF-κ B 蛋白的表达情况。结果：83例肺癌中，RKIP蛋白的低表达率及NF-κ B 蛋白的高表达率分别为20.5%（17/83）、68.7%（57/83），其表达水平与肺癌的分化程度、TNM分期、淋巴结或远处转移及术后生存时间有明显关系（P 均<0.05）；RKIP蛋白的表达水平与NF-κ B 呈负相关（P<0.05）；RKIP蛋白低表达与NF-κ B 蛋白高表达的病例平均生存时间和5 年生存率明显降低（P<0.05）。 结论：RKIP低表达与肺癌的侵袭、转移密切相关，RKIP可能是肺癌的一个转移抑制蛋白，RKIP表达下调可能通过活化NF-κB 信号通路促进肺癌细胞的侵袭和转移。Abstract: Objective:To investigate the expression of raf kinase inhibi tor protein (RKIP) and NF-κ B in lung carcinoma and its correlation with clinicopathologic features and survival time. Methods:RKIP and NF-κ B protein levels were detected using immunohistochemistry in 83lung carcinoma specimens and 65non-tumor lung samples.Results: Immunohistochem-istry revealed that of the 83carcinoma cases, there was low expression of RKIP and high expression of NF-κ B protein, 20.5% (17/83) and 68.7% (57/83), respectively. Expression levels of RKIP and NF-κ B protein were correlated wi th tumor differentiation, TNM staging, lymph node metastasis, and survival time (P<0.05, P<0.05, P<0.05, P<0.05, respectively). RKIP expression was negatively correlated wi th NF-κ B expression in lung carcinoma (P<0.05). The mean survival time in cases with a low expression level of RKIP protein was significantly shorter than those with a high expression level, whereas the mean survival time in cases wi th a high expression level of NF-κ B protein was signi ficantly shorter than those wi th a low expression level ( P<0.05). Conclusion: Expression of RKIP and NF-κ B are closely correlated wi th invasion and metastasis of lung carcinoma. RKIP may be a lung carcinoma metastasis suppressor, and decreased RKIP expression is associated wi th the increased metastatic potential of lung carcinoma cel ls through the activation of the NF-κB signal ing pathway.
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