摘要:
探讨垂体肿瘤转化基因（PTTG）、碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子-C（VEGF-C）在大肠癌中的表达及以上指标与大肠癌临床病理因素之间的关系。方法：采用RT-PCR技术检测20例大肠正常黏膜、80例大肠癌组织中PTTG mRNA，应用免疫组织化学法检测20例大肠正常黏膜、80例大肠癌组织中PTTG、bFGF、VEGF-C蛋白的表达。结果：大肠癌组织中PTTG、bFGF、VEGF-C的表达明显高于大肠正常黏膜组织，且与大肠癌浸润深度、淋巴结转移、Duke's分期呈正相关，PTTG、bFGF、VEGF-C在大肠癌中的表达有显著正相关性。结论：PTTG、bFGF、VEGF-C在大肠癌发生、发展、侵袭及转移过程中扮演着重要的角色，可作为判断大肠癌转移及预后的预测因素，且三者可能通过相互作用共同促进大肠癌的恶性进展。
Abstract:
investigate the expression and clinical significance of the pituitary tumor transformed gene ( PTTG ), the basic fibroblast growth factor ( bFGF ), and the vascular endothelial growth factor-C ( VEGF-C ) in the occurrence and progression of human colorectal carcinoma ( CC ). The relationship between these indices and clinicopathologic factors was also examined. Methods: Reverse transcriptase-polymerase chain reaction ( RT-PCR ) was used to determine the mRNA expression of PTTG. Streptavidin peroxidase ( SP ) immunohistochemical staining was performed to assay the expression of the three proteins in 20 normal mucosa ( NM ) and 80 CC tissues. Results: The PTTG, bFGF, and VEGF-C expression levels in CC were significantly higher than those in NM ( P < 0.01, P < 0.01, and P < 0.01, respectively ), and were positively correlated with the depth of tumor invasion, lymph node metastasis, and Duke's staging. Significantly positive correlations were observed among PTTG, b-FGF, and VEGF-C in CC tissues. Conclusion: The high PTTG, bFGF, and VEGF-C expression may be significantly involved in the onset and progression of CC, and can be used as a predictive and prognostic factor. Interactions between PTTG, bFGF, and VEGF-C may promote CC progression.
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