Abstract: Objective :To study the expression status of Pin1,its association with Ki67 and their clinical importance in cervical cancer. Methods :Amplification of Pin1 gene was examined by RT-PCR,and the expression of both Pin1 and Ki67 protein were examined by immunohistochemistry in cervical cancer tissues. Results :1.A significantly higher expression of Pin1 mRNA was detected in cervical cancer than those in normal cervical tissues(P<0.05).2.The expression of Pin1 increased progressively along with the disease process from normal cervix to CIN and to cervical cancer(P<0.05).3.No significant difference of Pin1 expression was found between disease stage(FIGO),pathological grade or pelvic lymph node metastasis status(P>0.05).But the expression of Pin1 was significantly higher in adenocarcinoma than those in squamous carcinoma of the uterine cervix(P<0.05).4.In cervical cancer,the overexpression of Pin1 was positively correlated with that of Ki67(P<0.05). Conclusion :The overexpression of Pin1 is closely related to cancer cell proliferation or progression of cervical cancer and contributed to oncogenesis.Pin1 may serve as a potential marker for cervical cancer diagnosis.